Home / Science / People / Papers / Antibodies / CBMs / Protocols / Galleries

 

 

 

Micrograph Galleries - Gallery 1

Gallery 2 / Gallery 3 / Gallery 4 / Gallery 5

/ Gallery 6

_______________________________________

 

1) TS floral stem of Arabidopsis immunofluorescently labelled with monoclonal antibody JIM5 (homogalacturonan). Resin-embedding. jpk 1990

2) The same section as (1) showing Calcofluor White staining of cellulose. Note secondary walls of the sclerified parenchyma. jpk 1990

3) Three intercellular spaces, showing space formation, in pea stem parenchyma (TS) labelled with Calcofluor White for cellulose (blue) and monoclonal antibody LM7 for non-blockwise-deesterified homogalacturonan (green). Hand-cut sections. wgtw 2001 ref

 

4) Immunolabelling of TS tobacco stem parenchyma with anti-homogalacturonan (HG)  phage monoclonal PAM1 (used in soluble scFv format). PAM1 binds to blocks of de-esterified HG and the epitope is abundant at the lining of intercellular spaces and also occurs at pit fields. Hand-cut section. wgtw 2001 ref

5) Intercellular space between 3 pea stem parenchyma cells (TS). Blue is Calcofluor White labelling of cellulose, and the red and green indicate immunolabelling of diverse patterns of methyl-esterification of homogalacturonan by LM7 and PAM1 respectively. Hand-cut section. wgtw 2001 ref

 

5) Medial LS carrot root apex immunofluorescently labelled with anti-(1→4)-b-D-galactan monoclonal antibody LM5. Here, as in many organs/species, the LM5 epitope appears in cell walls during cell development. Cryosection. jpk1999 ref

 

 

7) Equivalent medial LS carrot root apex to (6) immunofluorescently labelled with anti-(1→5)-a-L-arabinan monoclonal antibody LM6. Cryosection. jpk1999 ref

 

 

8) Internal face of tomato pericarp cell wall immunofluorescently labelled with anti-homogalacturonan JIM5. The JIM5 epitope is abundant at pit fields. co 1999 ref

 

9) Internal face of tomato pericarp cell wall immunofluorescently labelled with anti-galactan monoclonal antibody LM5. The LM5 epitope is absent from pit fields. co 1999 ref

 

_______________________________________