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Micrograph Galleries - Gallery 3

Gallery 1 / Gallery 2 / / Gallery 4 / Gallery 5

/ Gallery 6

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1) TS of lupin seed cotyledon parenchyma immunofluorescently labelled using monoclonal antibody LM5 (pectic galactan) showing thickened cell walls around intercellular spaces at cell junctions. Resin-embedded material. lm 2000 ref

2) Similar TS of lupin seed cotyledon parenchyma as (1) immunofluorescently labelled using monoclonal antibody LM6 (pectic arabinan) showing that this epitope is restricted to non-thickened cell wall regions/middle lamella. Resin-embedded material. lm 2000 ref

3) Tissue print on nitrocellulose of cut surface of a lemon (TS) probed with monoclonal antibody LM5 (pectic galactan) which indicates that this polymer is abundant in the endocarp extensions forming the segments. wgtw 1998 ref

4) Tissue print on nitrocellulose of a cut surface of a lemon (TS) probed with monoclonal antibody LM6 (pectic arabinan) which indicates that this polymer is abundant in the endocarp extensions forming the juice sacs. wgtw 1998 ref

5) TS pea epicotyl immunofluorescently labelled with a monoclonal antibody to amine oxidase, LM4, showing the abundance of this enzyme in cell walls at intercellular spaces. Note absence from epidermal cell walls. Hand-cut section.  jpk1998 ref ref

 

6) Equivalent TS pea epicotyl to (5) immunofluorescently labelled with LM5, showing the abundance of pectic galactan in parenchyma cell walls but low levels in radial epidermal cell walls.  jpk1998

7) Equivalent TS pea epicotyl to (5)  immunofluorescently labelled with LM6, showing pectic arabinan. Note abundance of the LM6 epitope in radial cell walls of epidermal cells.  jpk1998

 

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