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Micrograph Galleries - Gallery 4

Gallery 1Gallery 2 / Gallery 3 / / Gallery 5

/ Gallery 6

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1) An intact Arabidopsis seedling root immunolabelled with anti-pectic galactan LM5 indicating the developmental dynamics of this epitope. The epitope is most abundant at a region that marks the onset of the rapid phase of cell elongation. x marks the most distal point of the root tip. lm 2002 ref

 

2) Combination of bright field and LM5 immunofluorescence labelling of an intact Arabidopsis seedling root showing how the band of pectic galactan at the root surface marks the transition to cell elongation. Root tip to the right of the micrograph. jpk 2002 ref

 

3) An intact Arabidopsis seedling root immunolabelled with anti-arabinan LM6 indicating the developmental dynamics of this epitope. lm 2002

4) Whole mount preparation of the moss Physcomitrella patens immunolabelled with JIM5 showing the presence of the homogalacturonan epitope at all protonemal surfaces. kjdl ref

 

5) Whole mount preparation of the moss Physcomitrella patens immunolabelled with LM6 showing the presence of arabinogalactan-proteins at the growing tip of protonemal filaments. kjdl  ref

 

6) Excised piece of lupin testa immunolabelled with anti-xylogalacturonan LM8 showing specific occurrence of this pectic polymer in detaching inner parenchyma cells. wgtw 2000 ref

 

7) Resin-embedded section through a pea root apex immunolabelled with anti-xylogalacturonan LM8 showing occurrence of this pectic epitope in cell walls of outer root cap cells that are destined for detachment. cgsk 2000 ref

 

8) Same section as 4) stained with Calcofluor white showing all cell walls.

9) Whole mount preparation of a maize root apex FITC-immunolabelled with anti-xylogalacturonan LM8 showing occurrence of this polymer in the mucilage surrounding the apex. Note separated root cap cells in the mucilage. jpk 2000 ref

 

10) Section of resin-embedded maize root cap stained with calcofluor to indicate cellulose (blue) and FITC-immunolabelled with anti-xylogalacturonan LM8 showing occurrence of this polymer in the outer cells of the root cap. cgsk 2000 ref

11) Medial longitudinal section of resin-embedded root apex of Arabidopsis (Landsberg erecta ecotype) FITC-immunolabelled with anti-xylogalacturonan LM8. cgsk 2000 ref

 

 

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