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Hemp I / Hemp II / Asplenium / Ferns / Xyloglucan / Xylan / Arabinan / Cell adhesion

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Asplenium:

Immunofluorescence detection of cell wall epitopes in transverse sections of petioles of the fern Asplenium aethiopicum.
Further details:
Leroux et al. 2007

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TS of petiole of fern Asplenium aethiopicum showing vascular bundle and surrounding cortical parenchyma. Cycling images of Calcofluor staining, LM6 arabinan epitope occurrence and combined image along with red wavelength autofluorescence.

 

Region of stele and surrounding parenchyma in Asplenium petiole showing LM10 xylan epitope in xylem vessel cell walls 

 
 

The JIM5 homogalacturonan epitope is abundant in the intercellular pectic protuberances seen at intercellular spaces of the petiole parenchyma cells. Blue fluorescence shows primary cell walls

 

The PAM1 homogalacturonan epitope in is also abundant in the extensive pectic protuberances seen in intercellular spaces. Blue fluorescence shows primary cell walls

 

View of whole TS of petiole of fern Asplenium aethiopicum showing that outer cortical parenchyma layers do not stain with Calcofluor White

 

 

   

 

   

Last modified 22/02/10