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Hemp I / Hemp II / Asplenium / Ferns / Xyloglucan / Xylan / Arabinan / Cell adhesion

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Xyloglucan:
non-masked & un-masked

Xyloglucan detection in cell walls with monoclonal antibody LM15. Right: Cycling images of xyloglucan (FITC) & Calcofluor fluorescence showing thickened cell walls of tamarind seed cotyledon parenchyma. We have discovered that, in some cases, xyloglucan epitopes can be effectively masked by pectic homogalacturonan in many plant cell walls. For details see Marcus et al. 2008

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Un-masked xyloglucan. Pectate lyase treated TS of pea stem showing complex pattern of the un-masked LM15 xyloglucan epitope in relation to a vascular bundle (FITC) combined with Calcofluor fluorescence of all cell walls (blue). 

 
 

Non-masked xyloglucan. TS of pea stem showing the LM15 xyloglucan epitope is most abundant in vascular bundles. No enzymatic pre-treatment of section.

 
 

Un-masked xyloglucan. An equivalent TS to that above showing the occurrence of the LM15 xyloglucan epitope after section pre-treatment to remove pectic homogalacturonan. The epitope is now widely detected in epidermal cells walls and parenchyma systems.

 
 

Unmasked LM15 xyloglucan epitope as seen in TS pith parenchyma of tobacco. Section pretreated to remove pectic homogalacturonan prior to antibody incubation. The epitope is most abundant at the junctions between adhered and non-adhered cell walls at intercellular spaces.

 

TS of cotyledon parenchyma of nasturtium (Tropaeolum majus) seeds. Cycling images of Calcofluor staining and the FITC-tagged  LM15 xyloglucan epitope in cell wall regions close to middle lamellae. No enzymatic pre-treatment of section.

 
       
     

 

 

 

 

   

 

   

Last modified 22/02/10