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Hemp I / Hemp II / Asplenium / Ferns / Xyloglucan / Xylan / Arabinan / Cell adhesion

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Arabinans revealed

The detection of arabinan and related polysaccharides in both primary and secondary cell walls of a range of systems has been carried out with two new monoclonal antibodies LM13 and LM16 in addition to the established arabinan probe LM6. LM13 binds to linearised arabinans and LM16 to the product of arabinofuranosidase action.

Right: LM6 binding to Equisetum sclerenchyma cell walls.

For details see Verhertbruggen et al. 2009

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Monoclonal antibody LM6 binding to a TS of Arabidopsis thaliana stem. This antibody, that recognizes a small epitope of 1,5-arabinan, binds to most cell walls.

 
 

Equivalent TS of Arabidopsis thaliana stem to above immunolabelled with monoclonal antibody LM13. This antibody, requiring more arabinosyl residues for recognition, has preference for unbranched 1,5-arabinans and in this organ the epitope is restricted to epidermal cell walls.  

 
 

Pair of micrographs showing monoclonal antibody LM16 binding to cortical / xylem region of a transverse sections of a tobacco stem (surface to left).

Upper panel: untreated section.

Lower panel: equivalent section pre-treated with an arabinofuranosidase.

The enzyme treatment results in the LM16 epitope being detected in most cell walls and not just subsets of phloem and xylem cells.

 
 

Micrograph showing monoclonal antibody LM13 binding (FITC) to subsidiary guard cell wall thickenings in a pair of stomata in a TS of Equisetum ramosissimum stem. Blue shows Calcofluor labelling of all cell walls.

 

Equivalent section of Equisetum ramosissimum stem as shown above showing a double stomatal system - immunolabelled with monoclonal antibody LM16 and revealed by FITC fluorescence. In this case LM16 binds to one pair of guard cells only.

 
       
     

 

 

 

 

   

 

   

Last modified 22/02/10