One of our research aims is
to generate defined probes for the analysis of cell wall glycans in situ.
Probes such as monoclonal antibodies to oligosaccharide epitopes are crucial
tools to dissect the temporal and spatial occurrence of cell wall
polysaccharides and their modifications during the life of a plant.
Moreover, they are essential tools for determining the polymer arrangements
and architecture of individual cell walls. Our
probes are generally rat hybridoma or phage display monoclonal antibodies. We
are also exploring the capacity of recombinant his-tagged
carbohydrate-binding modules (CBMs) from cell wall
to act in a similar manner as antibodies as probes for cell wall components.
The generation of monoclonal
antibodies to glycans is not straightforward. The limitations include the
low immunogenicity of glycans which often necessitates the preparation of neoglycoprotein immunogens.
This involves the coupling of a defined oligosaccharide to an
immunogenic protein such as BSA. This strategy has led to the generation of
monoclonal antibodies to 1,4-galactan,
1,5-arabinan and xyloglucan. A compounding factor is the limited
availability of appropriate defined oligosaccharides that are required both
for the coupling to protein and immunogen preparation and also for the
characterization of derived antibodies.
If you are interested in the
generation of monoclonal antibodies to a specific plant cell wall polymer or
set of polymers and may have useful resources to achieve this please contact
us. Monoclonal antibodies in which we have been involved in generating are
listed here: ANTIBODIES.
describing the supply of antibodies from our laboratory
antibody data base
Database of monoclonal antibodies and other
resources for plant cell walls.
antibodies for plant cell walls
Web page detailing NSF-funded antibody generation
project at the Complex Carbohydrate Research Center (CCRC), Georgia, USA
The CCRC can also supply JIM5, JIM7 and an expanding range of other
cell wall monoclonal antibodies.